stat3 protein  (MedChemExpress)

Journal: bioRxiv

Article Title: Tirzepatide Synergizes with Leptin on Weight Loss and Restoring Metabolic Homeostasis in Diet-induced Obesity Model

doi: 10.64898/2025.12.18.695152

Figure Lengend Snippet: Veh = vehicle, Lep = leptin (LY355101), TZP = tirzepatide, PF = pair-feeding, SC = subcutaneous, POMC = pro-opiomelanocortin, ARH = arcuate nucleus of hypothalamus, GLP1-R = glucagon-like peptide-1 receptor, pSTAT3 = phosphorylated STAT3. (A) Schematic illustration of the study design (6 dosing groups: Veh + Veh, Veh + Lep, TZP + Veh, TZP + Lep, PF + Veh, PF + Lep). n = 8 per group. (B) Representative images of Agrp and Pomc positive cells co-expressing pSTAT3 protein. (C) Representative images of Glp1r+, Gipr+ cells and pSTAT3 protein expression. (D) Percentage of Pomc+/pSTAT3+ cells out of all Pomc+ cells in ARH, calculated as the average of 3 sections. n = 7-8. Dunnett’s test one-factor linear model. # P < 0.05 compared to TZP/Veh. (E) Percentage of Glp1r+/pSTAT3+ cells out of all Glp1r+ cells in ARH, calculated as the average of 3 sections. n = 7-8. Dunnett’s test one-factor linear model. ## P < 0.01 compared to TZP/Veh. (F-H) qPCR analysis of Socs3, Pomc, and Npy in the hypothalamus from DIO mice after 3 weeks of reagent treatment. n = 6-9 per group. One-way ANOVA. *p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. Data are displayed as means ± SEM.

Article Snippet: Formalin-fixed paraffin-embedded brain sections (3 sections per mouse per triplex) underwent RNAscope in situ RNA hybridization (ISH) for the detection of Lepr (ACDBio probe #418858-C2), Glp-1r (ACDBio probe #418858-C2), Gipr (ACDBio probe #319128-C1), Pomc (ACDBio probe #314088-C2) and Agrp (ACDBio probe #400718-C1), followed by immunofluorescence (IF) staining for pSTAT3 protein (Cell Signaling anti-pSTAT3 #9145).

Techniques: Expressing

Journal: bioRxiv

Article Title: Tirzepatide Synergizes with Leptin on Weight Loss and Restoring Metabolic Homeostasis in Diet-induced Obesity Model

doi: 10.64898/2025.12.18.695152

Figure Lengend Snippet: LepR = leptin receptor, GLP-1R = glucagon-like peptide-1 receptor, pSTAT3 = phosphorylated STAT3, Pomc = pro-opiomelanocortin, Agrp = Agouti-related peptide, EX4 = Exendin4. (A) Representative in situ images of Lepr, Glp1r, and Pomc positive cells in arcuate nucleus of hypothalamus. Scale bar: 100um. (B) Bar graph quantification of the overlapping Lepr/Glp1r/Pomc out of total Pomc (%). n = 3. (C) Representative in situ images of Lepr, Glp1r, and Agrp positive cells in arcuate nucleus of hypothalamus. Scale bar: 100um. (D) Bar graph quantification of the overlapping Lepr/Glp1r/Agrp out of total Agrp (%). n = 3. (E-G) The dose-response curves of pSTAT3 induction by leptin co-treated with fixed concentration of EX4 (0 nM, 1 nM, 10 nM, respectively) in HEK293 cells transfected with human LepR/GLP1R (E), LepR alone (F), or GLP1R alone (G). N = 3 for each leptin dose level. Data are displayed as means ± SEM.

Article Snippet: Formalin-fixed paraffin-embedded brain sections (3 sections per mouse per triplex) underwent RNAscope in situ RNA hybridization (ISH) for the detection of Lepr (ACDBio probe #418858-C2), Glp-1r (ACDBio probe #418858-C2), Gipr (ACDBio probe #319128-C1), Pomc (ACDBio probe #314088-C2) and Agrp (ACDBio probe #400718-C1), followed by immunofluorescence (IF) staining for pSTAT3 protein (Cell Signaling anti-pSTAT3 #9145).

Techniques: In Situ, Concentration Assay, Transfection